University of Technology SydneyUTS:Faculty of Science
Microstructural Analysis Unit



Staff
Research
Facilities and Equipment
Services
Home

Faculty Home
Search
Index
Sitemap


Varian Cary Eclipse Fluorescence Spectrometer

Eclipse Spectrophotometer

  • Operates in fluorescence, phosphorescence or bio/chemi-luminescence modes
  • Xe pulse lamp source (80 Hz)
  • Czerny-Turner monochromators
  • Excitation and emission wavelength range of 200-900 nm (High performance R928 PMT)
  • Minimum slit width of 1.5 nm
  • Scan rates from 0.01-24 000 nm/min, 80 data points per second maximum measurement rate
  • Cary Eclipse Bio Pack software package, operates in following modes:
      • Scan Application
        Excitation, emission and synchronous scans available with 3D data collection available in all scanning modes. 3D scans can be exported into Grams 3D or represented as contour plots.
      • Simple Reads
        Performs single wavelength intensity readings of samples
      • Advanced Reads
        Can be set up to take multiple readings of a sample to obtain a mean measurement
      • Ratio Application
        This is used to measure changes in intensity at two different wavelengths - this is useful for monitoring wavelength shifts in dyes whose wavelength maxima change when bound to ions.
      • Kinetics Application
        Measures changes in emission intensity as a function of time
      • Lifetimes Application
        Measures the phosphorescence lifetimes by measuring the average time that a molecule remains in the excited state following excitation
      • Concentration Application
        Used to determine the concentration of an absorbing sample by first acquiring a calibration curve of up to 30 points
  • Standard cell holder and solid sample holder accessories available (pictured below)

Liquid cell holder

Solid sample holder

Standard cell holder, quartz cell available for use.

Solid sample holder accessory with powder holder attached. Other accessories for solid samples available.