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Adipocere Research Program

Shari Forbes, B.Sc. Ph.D.
Centre for Forensic Science
University of Western Australia
35 Stirling Highway,
CRAWLEY WA 6909
Australia
Ph: int +61 8 9380 3408
Fax: int +61 8 9380 7285
Email: sforbes@cyllene.uwa.edu.au


Background

In 2000 Shari commenced research towards her doctoral degree at the University Of Technology, Sydney which was funded by an APA postgraduate scholarship. The research extended on her previous work conducted into adipocere formation in grave soils. Her doctoral research aimed to investigate the factors which influence the formation of adipocere in a burial environment. These factors included soil type, pH, moisture, temperature, lime, presence of clothing and presence of a coffin. Samples of adipocere and grave soils were collected from human exhumations conducted throughout Australia. Decomposition trials utilizing pigs as human models also allowed the collection of adipocere and soil samples. In order to determine those factors which affect adipocere formation, controlled laboratory experiments were carried out which formed a model adipocere product. Additionally, experiments investigating the formation of adipocere on different animal species were conducted.

The study was successful in identifying trace levels (ppm) of adipocere in grave soils and the ability of adipocere to leach into soil. The similarity in chemical composition between human and pig adipocere samples allowed for the use of pig adipose tissue in the model adipocere experiments. The study identified those factors which are conducive to adipocere formation and whether the individual factor accelerated or retarded adipocere formation when compared to a control. The experiments were able to form adipocere on numerous animal species but could not distinguish species type by chemical composition. The results were reported in a thesis entitled 'An Investigation of the Factors Affecting the Formation of Adipocere in Grave Soils'. Several publications in a diverse range of journals resulted from her research. Shari completed her doctoral degree in February 2003.


Current and Future Research

Shari is now employed at the Centre for Forensic Science at the University of Western Australia where she is continuing her research into decomposition and adipocere. An important question that has arisen from her research is whether adipocere can be used to determine postmortem interval (PMI) of decomposing remains. Continual research into this area is necessary to determine whether adipocere could feasibly be used to determine time since death. Numerous decomposition environments will be explored and will include many different factors additional to those investigated for her doctoral degree.

The extraction of DNA from adipocere is currently being attempted to determine the extent of degradation that occurs during decomposition. The possibility of using DNA degradation as a technique for determining PMI will be concurrently investigated. Additionally, species identification using DNA extracted from adipocere will be explored and will hopefully overcome the limitations of chemical composition identified in Shari's previous research.

Shari's research will continue to focus on PMI determination. She aims to explore additional techniques and disciplines in which alternative methods for determining PMI may be identified. Her experience in the discipline of forensic chemistry will be extended to incorporate the fields of forensic entomology and forensic anthropology. She will investigate the entomology associated with various decomposition environments and the feasibility of using nitrogen isotope concentration in fly larvae to determine time since death. Radioactive isotope analysis of bone will be explored to date anthropological remains of forensic importance.

Shari will continue to collaborate with UTS on honours projects and decomposition research with Dr Barbara Stuart and Dr Boyd Dent.


Publications

Refereed Journal Articles
Forbes SL, Dent BB, and Stuart BH, 2003, The effect of soil type on the formation of adipocere in grave soil, Forensic Science International, Submitted.

Dent BB, Forbes SL, and Stuart BH, 2003, Review of human decomposition processes in soil, Journal of Environmental Geology, Submitted.

Forbes SL, Keegan J, Stuart BH, and Dent BB, 2003, Development of a GCMS method for the detection of adipocere in grave soils, European Journal of Lipid Science and Technology, Submitted.

Forbes SL, Stuart BH, Dadour IR, and Dent BB, 2002, A quantitative investigation of pig adipocere, Journal of Forensic Science, In revision.

Forbes SL, Stuart BH, and Dent BB, 2002, The identification of adipocere in grave soils, Forensic Science International, 127, 225-230.

Stuart BH, Forbes SL, Dent BB, and Hodgson G, 2000, Studies of adipocere using diffuse reflectance infrared spectroscopy, Vibrational Spectroscopy, 24, 233-242.


Conference Papers
Forbes SL, 2002, An Investigation of the Formation of Adipocere in Grave Soils, 16th International Symposium on the Forensic Sciences, Canberra, 12–17 May, Forensic Sciences - Outcomes for Society Handbook, 162-163.


Links

http://www.forensicscience.uwa.edu.au

http://www.forensics.com.au

http://www.agric.wa.gov.au/ento/forensic.htm