Courses and Training

The PTCE over a wide range of courses from one day specific courses, three day training courses, five day intensive courses and long term “research hotel” training. Courses can be tailored to individual researcher’s sample and attendees are encouraged to bring their own samples.

Sample preparation for isoelectric focusing (one day).

Basic principles of sample prep for gel electrophoresis (Tissue grinding, sonication, bead milling, etc).

Desalting

Sequential extraction techniques

Micro-organism extraction

Membrane enrichment techniques

Protein assays

Reduction and alkylation

Isoelectric focusing with IPG strips

Sample preparation and two-dimensional electrophoresis course (three days).

*Day 1:*	Basic principles of sample prep for gel electrophoresis (Tissue grinding, sonication, bead milling, etc). Desalting. Micro-organism extraction. Membrane enrichment techniques. Protein assays. Reduction and alkylation. Isoelectric focusing with IPG strips. Liquid phase electrophoretic fractionation (Multicompartment electrolysers and Rotofor). Prep Cell.
*Day 2:*	Equilibration of IPGs for second dimension electrophoresis. Native and denaturing gels. buffer systems - stacking theory. Gel Blotting to membranes. Protein staining with visible stains, fluorescent stains and western blotting.
*Day 3:*	Gel imaging and spot detection with Proteomeweaver and PDQuest. Experimental design for differential display and DIGE experiments. Troubleshooting.