Proteomics

What is Proteomics


Proteomics is determining the entire protein content of a particular sample, be it a microorganism, cultured cell line, organelle, tissue or whole organism. While the genome of an organism is rather constant, the proteome can differ from cell to cell and change constantly as the cell interacts with its own genome and the environment. In addition, proteins undergo post-translational modifications and their interactions with other proteins effect cells in ways not predicted by the genome. The Human Genome Project has found that there are far fewer protein-coding genes in the human genome than proteins in the human proteome (20,000 to 25,000 genes vs. > 500,000 proteins). The protein diversity is thought to be due to alternative splicing and post-translational modification of proteins. The discrepancy implies that protein diversity cannot be fully explained by genomics and proteomics is then necessary to determine what proteins are expressed and how they are regulated in different states of the cell. The main tools of proteomic projects are two-dimensional gel electrophoresis and multi-dimensional chromatography techniques coupled to mass spectrometry. What is the PTCE? The PTCE is one of five Centres of Expertise within the Faculty of Science at UTS. The Centres of Expertise provide focus and concentration for technical skills and services in which UTS excels. The PTCE’s goals are to: Provide a world class proteomics facility for UTS researchers. Establish UTS as a world leader in proteomics and technology development Attract collaborations and seek funding opportunities through Australian and international agencies Develop new proteomics technologies to enable key projects within UTS Assemble a strong IP portfolio centred on key technologies to underpin UTS projects Partner with industry to commercialise technology and access the latest technologies and trends Provide a showcase facility for industry partners Provide intra-university training for existing researchers as well as honours and postgraduate students What can the PTCE do for my research? The PTCE brings internationally recognised expertise in technology development for sample preparation, fractionation and two dimensional gel electrophoresis. The PTCE also has growing expertise in multi-dimensional chromatography and mass spectrometry. PTCE staff can review and advise researchers on how the PTCE can help them achieve their research goals. The PTCE will also provide detailed training in sample preparation techniques, gel-based separation technologies, gel imaging techniques, protein spot excision and preparation of isolated spots for mass spectrometry. Subsequent to training, researchers will be able to access all of the PTCE’s technology and equipment. Consumables will be provided by the PTCE on a cost recovery basis. To put it simply, the PTCE will provide the training, resources and equipment researchers need while the researchers need to provide a pair of hands and a sample.

What is the PTCE?

The PTCE’s goals are to:

What can the PTCE do for my research?